Main Researcher: Jakob Albrethsen
Other Researchers: Hanne Frederiksen, Anders Juul, Anna-Maria Andersson
Objective: To develop a novel assay for quantitation of INSL3 as a marker of Leydig cell function and to study the relationship between exposure to endocrine disrupting chemicals (EDCs) and INSL3.
Description: Endocrine disrupting agents are known to affect the embryological development and function of male gonads. INSL3 is expressed exclusively by the Leydig cells of the testicles and play a key role in development of male gonads. A few animal studies have reported changes in INSL3 expression in response to exposure to endocrine disrupting agents. In extent of previous preliminary results, we aim to determine the potential role of INSL3 as a biomarker for exposure to endocrine disrupting agents in humans.
Outcome: A mass spectrometry-based assay for serum INSL3 has been developed and validated.
Output: So far, INSL3 has been quantitated in 200 human serum samples for assay validation and to establish the normal range of INSL3 in human serum.
Publications: Manuscript presenting the analytical method is in preparation