UV filters in seminal fluid, serum and urine from young Danish men - EDMaRC

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UV filters in seminal fluid, serum and urine from young Danish men

Main Researcher: Hanne Frederiksen

Other Researchers:  Anna-Maria Andersson, Niels Jørgensen and Niels Erik Skakkebæk
- To elucidate the extent to which human sperm cells are directly exposed to UV filters via their presence in seminal fluid.
- To elucidate the distribution of UV filters in seminal fluid, serum and urine samples to a) increase our knowledge on distribution of these substances in the body and b) to investigate if prospectively it will be possible to provide a qualified estimate of exposure of sperm cells in the seminal fluid based on measurements in urine or serum.
- To elucidate if UV filter exposure could be associated with biological endpoints such as the level of male reproductive hormones and semen quality. 
Description: In total nine chemical UV filters; benzophenone (BP), benzophenone-1 (BP-1), benzophenone-2 (BP-2), benzophenone-3 (BP-3), 5-cholro-2-hydroxybenzophenone (BP-7), 4-hydroxybenzophenone (4-HBP), 4-methyl-benzophenone (4-MBP), 3-(4-methylbenzylidene)-camphor (4-MBC) and 3-benzylidene camphor (3-BC) were analyzed by TurboFolow-LC-MS/MS in urine, serum and seminal fluid samples from 309 young Danish men from the general population; for each man the sample types were collected within one hour. The samples were collected during February-December 2013.
Outcome:  BP-3 and BP-1 were detectable in nearly all urine samples and also present in the highest concentrations. BP and 4-HBP were detectable in almost half of the urine samples. The other UV filters; BP-2, BP-7, MBP-4, 4-MBC and 3-BC were either not detectable or only measurable in very few samples and in very low concentrations in the urine. The most frequently detectable UV filters in serum were 4-HBP, 4-MBP and BP-3 and in seminal fluid BP, BP-1, BP-3 and 4-MBP were detectable in a subset of samples.
Status: Ongoing statistical analyses will in nearest future show whether UV filter concentrations measured in serum and urine could be correlated with levels observed in seminal plasma and thereby be a proxy for direct semen cell exposure. Furthermore the statistical analyses of UV filter exposure associated to biological outcomes are initiated.
Publications: in preparation
This project has been co-funded by Center for Endocrine Disruption (CeHoS)
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